Research conclusions:(1)Currently there are many global differences in research results，conclusions and viewpoints on biological effects of electromagnetic radiation; there are still uncertainties of the impact of electromagnetic radiation from high-speed railway on the human body, which needs long-term and in-depth exploration and research. (2) In general electromagnetic radiation from Wuhan-Guangzhou Passenger Line does not constitute prominent environmental impact ,but taking into account the emotions of anxieties and worries of the people along the line to electromagnetic radiation , the project construction company still makes its best efforts to take necessary protective measures to ensure that the health of residents around the sensitive facilities are not affected，and the people along the line whose TV reception may be affected receive reasonable compensation.(3)The mitigation measures include base station location optimization，housing function replacement and cost compensation，etc. (4) The research results of this paper will provide a reference for environmental impact assessment ( EIA) and preventive measures of the electromagnetic radiation from high-speed railway.